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The 22-nt long mature miRNA strand obtained from the intermediate duplex is then loaded into the Argonaute-containing RNA-induced silencing complex (RISC) and target mRNA molecules by miRNA:mRNA sequence complementarity and negatively affects gene expression either through mRNA cleavage and degradation via the perfect base-pairing with target mRNA or translation repression via imperfect complementation with 3UTR of target mRNA (Llave et al., 2002

A vial stored at room temperature for 24 hours loses approximately 1520% potency

"It just stops